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In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
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In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
Capítulos
- 00:00Título
- 00:21Supscription Lock
- 00:56Introduction
- 01:49Injecting DNA and Electroporation
- 05:36Analyzing Neuronal Process Outgrowth
- 06:22Analysis of the Cell Types of Electroporated Neurons
- 07:50Conclusion
In utero electroporation is a valuable method for transfecting neuronal progenitor cells in vivo. Depending upon the placement of the electrodes and the developmental timepoint of electroporation, certain subsets of cortical cells can be targeted. Targeted cells can then be analyzed in vivo or in vitro for effects of genetic alteration.
Tags
In Utero ElectroporationPrimary Neuronal CultureGene FunctionSubset Of Cortical NeuronsNeurite OutgrowthGenetic AlterationsShRNACDNA ConstructsChemical TransfectionViral TransductionHeterogeneous Pool Of Cell TypesGlutamatergic NeuronsInhibitory NeuronsGlial CellsEmbryonic Rodent CortexDeep Layers Of The CortexSuperficial Layers Of The CortexDorsal-medial Regions Of The CortexVentral-lateral Regions Of The CortexQuantitative AnalysisStep-by-step Method
