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Genoom-brede analyse met behulp van chip Isoform-specifieke Gene targets te identificeren
JoVE Journal
Biologia
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JoVE Journal Biologia
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
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Genoom-brede analyse met behulp van chip Isoform-specifieke Gene targets te identificeren

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11:19 min

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July 07, 2010

DOI:

10.3791/2101-v

DOI:
10.3791/2101-v

•

11:19 min
July 07, 2010

•

1 Views
, , , , , ,
1Department of Biochemistry and Molecular Genetics,University of Illinois Chicago – UIC, 2Research Unit on Biomedical Informatics,Universitat Pompeu Fabra, 3Genome Technology Core,Whitehead Institute for Biomedical Research

Summary

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Hier presenteren we een chromatine immunoprecipitatie (ChIP) procedure voor genoom-brede locatie analyse van eiwit-isovormen die verschillen in een histon-bindend domein. We zijn toe te passen op ChIP-Seq analyse om de doelstellingen van het KDM5A/JARID1A/RBP2 histon demethylase te identificeren.

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Cite this Article

Beshiri, M. L., Islam, A., DeWaal, D. C., Richter, W. F., Love, J., Lopez-Bigas, N., Benevolenskaya, E. V. Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets. J. Vis. Exp. (41), e2101, doi:10.3791/2101 (2010).

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