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鳉胚胎和细胞移植嵌合体生成Dechorionation
JoVE Journal
Biologia
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JoVE Journal Biologia
Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras
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鳉胚胎和细胞移植嵌合体生成Dechorionation

17,340 Views

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09:03 min

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December 22, 2010

DOI:

10.3791/2055-v

DOI:
10.3791/2055-v

•

09:03 min
December 22, 2010

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28 Views
, ,
1Centre for Regenerative Medicine, Department of Biology and Biochemistry,University of Bath

Summary

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由于硬的蛋壳和软的胚胎，操纵鳉胚胎是比在斑马鱼中更多地参与。该视频显示一步一步的程序，包括dechorionation如何操作鳉胚胎，在琼脂糖凝胶成像和细胞移植嵌合体的生产安装。这些程序是必不可少的，在实验室中使用鳉和斑马鱼为脊椎动物的基因组功能基因清扫充分利用其互补性的特点。

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Porazinski, S. R., Wang, H., Furutani-Seiki, M. Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras. J. Vis. Exp. (46), e2055, doi:10.3791/2055 (2010).

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