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激光捕获显微切割技术的果蝇外周神经元

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Laser Capture Microdissection of Drosophila Peripheral Neurons

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激光捕获显微切割技术的果蝇外周神经元

DOI:

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12:02 min

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May 24, 2010

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Eswar Prasad R. Iyer², Daniel N. Cox²

¹Department of Molecular and Microbiology,George Mason University, ²Krasnow Institute for Advanced Study,George Mason University

Capítulos

00:00Título
01:18Introduction
01:53Preparing the Larvae
03:29Dehydration and Cuticle Removal from Frozen Larval Sections
05:13Laser Capture Microdissection
08:11RNA Isolation from LCM Derived Cells
09:29Representative Results
11:00Conclusion

Summary

Tadução automática

在这个视频文章中，我们目前的方法隔离单个或多个<em>果蝇</em> DA神经元使用红外线（IR）的捕获激光捕获显微切割（LCM）的类三龄幼虫。从孤立的神经元获得的RNA可以很容易地用于下游应用，包括QRT - PCR或芯片分析。

Tags

Laser Capture Microdissection Drosophila Peripheral Neurons Dendritic Arborization Da Neurons Molecular Mechanisms Class-specific Dendrite Morphogenesis RNA Isolation Techniques Magnetic Bead-based Cell Purification Fluorescent Activated Cell Sorting (FACS)RNA Binding Protein-based Strategies Laser Capture Microdissection (LCM)Spatial Precision QRT-PCR Microarray Expression Profiling Third Instar Larval Stage

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