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Assessment of Hsp70 Chaperone Activity Using Escherichia coli dnaK756 Cells
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Transformation af E. coli-celler til et komplementationsassay til vurdering af Hsp70-chaperonaktivitet
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Bioquímica
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JoVE Journal Bioquímica
Transformation of E. coli Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity

Transformation af E. coli-celler til et komplementationsassay til vurdering af Hsp70-chaperonaktivitet

Assessment of Hsp70 Chaperone Activity Using Escherichia coli dnaK756 Cells

DOI:
10.3791/201542-v

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03:31 min

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March 08, 2024

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, , ,
1Department of Biochemistry & Microbiology,University of Venda

Tags

Keywords: E. ColiDnaK756Complementation AssayHsp70 Chaperone ActivityHeat ShockPlasmid DNATransformationAgar PlatesAntibiotic SelectionSerial DilutionsPermissive And Non-permissive Growth TemperaturesRecombinant Protein ExpressionKPfKPf-V436F

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