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Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining
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Förberedelse av G1-synkroniserade celler för att mäta ssDNA-foci bildning vid genotoxisk stress
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Preparation of G1 Synchronized Cells to Measure ssDNA Foci Formation upon Genotoxic Stress
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Förberedelse av G1-synkroniserade celler för att mäta ssDNA-foci bildning vid genotoxisk stress

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07:11 min

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December 22, 2023

DOI:

10.3791/201062-v

DOI:
10.3791/201062-v

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07:11 min
December 22, 2023

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1Department of Biochemistry and Molecular Pharmacology,NYU Grossman School of Medicine, 2The Laura and Isaac Perlmutter Cancer Center,NYU Langone Health, 3Department of Cell Biology,NYU Grossman School of Medicine, 4Howard Hughes Medical Institute,NYU Grossman School of Medicine, 5Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences,HUN-REN Research Centre for Natural Sciences

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– G1 Synchronized Cells- SsDNA Foci Formation- Genotoxic Stress- Cell Synchronization- DNA Damage- DNA Repair

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Preparation of G1 Synchronized Cells to Measure ssDNA Foci Formation upon Genotoxic Stress. J. Vis. Exp. (Pending Publication), e201062, doi:10.3791/201062 (2023).

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