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A Guide to Precise Gene Disruption in Primary Bone Marrow-Derived Macrophages
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Förberedelse för elektroporering och ribonukleoproteinleverans för genomredigering i makrofager från musbenmärg
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Genética
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JoVE Journal Genética
Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages
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Förberedelse för elektroporering och ribonukleoproteinleverans för genomredigering i makrofager från musbenmärg

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04:03 min

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August 04, 2023

DOI:

10.3791/200896-v

DOI:
10.3791/200896-v

•

04:03 min
August 04, 2023

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1 Views
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1Department of Internal Medicine,University of California, Davis, 2Department of Medical Microbiology and Immunology,University of California, Davis

Tags

ElectroporationRibonucleoproteinGenome EditingMouse Bone Marrow-derived MacrophagesEDTAPBSCell CountCas9SgRNARibonucleoprotein ComplexCell SuspensionElectroporation Tip

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Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages. J. Vis. Exp. (Pending Publication), e200896, doi:10.3791/200896 (2023).

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