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Imunologia e Infecção

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用于生物能量研究的人外周血单核细胞的分离和冷冻保存

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies

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用于生物能量研究的人外周血单核细胞的分离和冷冻保存

162 Views

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02:46 min

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October 20, 2023

DOI:

10.3791/200888-v

DOI:

10.3791/200888-v

•

02:46 min

October 20, 2023

•

6 Views

Fabian Dieter, Jendrik Grube, Tim Birkenhauer, Alice Quentin, Gunter P. Eckert

¹Biomedical Research Center, Institute of Nutritional Sciences,Justus-Liebig-University of Giessen

Tags

Isolation Cryopreservation Peripheral Blood Mononuclear Cells PBMC Bioenergetic Studies DPBS Lymphocyte Isolation Medium Centrifugation Plasma Layer Resuspension Fetal Bovine Serum DMSO Cryotubes Freezing Liquid Nitrogen

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