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إنتاج فيروس النمط الكاذب SARS-CoV-2 ومقايسة تحييد المصل

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay

Please note that all translations are automatically generated. Click here for the English version.

إنتاج فيروس النمط الكاذب SARS-CoV-2 ومقايسة تحييد المصل

122 Views

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04:50 min

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November 21, 2023

DOI:

10.3791/200835-v

DOI:

10.3791/200835-v

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04:50 min

November 21, 2023

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7 Views

Tobia Fantoni*¹, Michele Bissoli*¹, Chiara Stefani, Mauro Voi, Alexandrina Dabija, Rebecca Casula, Domenico Luca Minafra, Julys da Fonseca Palmeira, Enrique Roberto Argañaraz, Martin Mayora-Neto, Nigel J. Temperton, Donato Zipeto, Alessandra Ruggiero

¹Department of Neuroscience, Biomedicine and Movement,University of Verona, ²Laboratory of Molecular Neurovirology, Faculty of Health Science,University of Brasília, ³Viral Pseudotype Unit, Medway School of Pharmacy,Universities of Kent and Greenwich at Medway

Tags

SARS-CoV-2 Pseudotype Virus Serum Neutralization Assay HEK 293T Cells Transfection Polyethylenimine Cell Culture Virus Production Viral Supernatant Serial Dilution HEK 293T ACE2 Cells Luciferase Assay Serum Samples Heat Inactivation

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