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Transendothelial Resistance Measurement to Assess Cell Barrier Integrity

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用于测量TEER的体外血脑屏障模型组装

JoVE Journal
Neurociência

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JoVE Journal Neurociência

In Vitro Blood-Brain Barrier Model Assembly for Measurement of TEER

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用于测量TEER的体外血脑屏障模型组装

Transendothelial Resistance Measurement to Assess Cell Barrier Integrity

DOI:

10.3791/200695-v

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03:39 min

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September 29, 2023

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Fuhan Fan, Hong Jiang, Ya Hou, Yi Zhang^2,3, Qipeng Zhao, Yong Zeng², Xianli Meng^2,5, Xiaobo Wang

¹School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine, ²Meishan Hospital of Chengdu University of Traditional Chinese Medicine, ³School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine, ⁴School of Pharmacy,Ningxia Medical University, ⁵Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline,Chengdu University of Traditional Chinese Medicine

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Keywords: In Vitro Blood-brain Barrier Model Assembly TEER Measurement BEND3 Cells DMEM Medium PET Membrane Cell Seeding Medium Exchange Electrode Calibration Resistance Measurement TEER Calculation Barrier Formation

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