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A 3D Blood-Brain Barrier Model Derived from Human Pluripotent Stem-Cells

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Создание 3D-гематоэнцефалического барьера, полученного из стволовых клеток человека in vitro

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Constructing the 3D Human-Stem-Cell-Derived In Vitro Blood Brain Barrier

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Создание 3D-гематоэнцефалического барьера, полученного из стволовых клеток человека in vitro

A 3D Blood-Brain Barrier Model Derived from Human Pluripotent Stem-Cells

DOI:

10.3791/200668-v

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02:08 min

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October 20, 2023

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Camille Goldman*^1,2,3,4,5, Natalie Suhy*^1,2,3,4,5, Jessica E. Schwarz^2,3,4,5, Emily Ruth Sartori^2,3,4,5, Rikki B. Rooklin^2,3,4,5, Braxton R. Schuldt^2,3,4,5, Louise A. Mesentier-Louro^2,3,4,5, Joel W. Blanchard^2,3,4,5

¹Icahn School of Medicine at Mount Sinai, ²Black Family Stem Cell Institute at Mount Sinai, ³Ronald M. Loeb Center for Alzheimer’s Disease at Mount Sinai, ⁴Friedman Brain Institute at Mount Sinai, ⁵Nash Family Department of Neuroscience at Mount Sinai

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Keywords: 3D Human-stem-cell-derived In Vitro Blood-brain Barrier IBBB Dissociated Endothelial Cells Pericytes Astrocytes Cell Mixture Cell Pellet Reduced GF Basement Membrane Matrix Cell Matrix Mixture 48-well Or 96-well Glass-bottom Culture Dish Polymerize The Matrix Supplemented Astrocyte Medium 96-well Plate Downstream Assays Properly Formed IBBB Single Translucent Disk Single Cells

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