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电刺激后平装大鼠视网膜的离体钙成像

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Ex Vivo Calcium Imaging of Flat-Mounted Rat Retina upon Electrical Stimulation

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电刺激后平装大鼠视网膜的离体钙成像

102 Views

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03:12 min

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August 18, 2023

DOI:

10.3791/200530-v

DOI:

10.3791/200530-v

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03:12 min

August 18, 2023

•

2 Views

Marina Cunquero, Maria Marsal, Gustavo Castro-Olvera, Steven T. Walston, F. Taygun Duvan, José Gabriel Macias-Montero, Carles Puigdengoles, Mokhtar Chmeissani, Jose A. Garrido⁴, Pablo Loza-Alvarez

¹Institut de Ciències Fotòniques (ICFO),The Barcelona Institute of Science and Technology, ²Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST,Campus UAB, ³Institut de Física d’Altes Energies (IFAE),The Barcelona Institute of Science and Technology, Campus UAB, ⁴Institució Catalana de Recerca i Estudis Avançats (ICREA)

Tags

Ex Vivo Calcium Imaging Flat-mounted Rat Retina Electrical Stimulation GCaMP 5G MEA AMS Medium Fluorescence Microscopy Pulse Generator Image Acquisition ImageJ ROI Photobleeching Correction

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