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甲醛交联 秀丽隐杆线 虫超声处理及染色质免疫沉淀

JoVE Journal
Genética

This content is Free Access.

JoVE Journal Genética

Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation

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甲醛交联秀丽隐杆线虫超声处理及染色质免疫沉淀

142 Views

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02:41 min

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May 05, 2023

DOI:

10.3791/200310-v

DOI:

10.3791/200310-v

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02:41 min

May 05, 2023

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1 Views

Chengyin Li*¹, Phoebe A. W. Bhagoutie*¹, Victor Lao, Arneet L. Saltzman

¹Department of Cell and Systems Biology,University of Toronto

Tags

Sonication Formaldehyde-Crosslinked Caenorhabditis Elegans Chromatin Immunoprecipitation Resuspension Buffer Detergents Lysate Centrifugation Immunoprecipitation Anti-H3K9 Trimethylation Anti-histone H3 IgG Protein G Magnetic Beads FA-150 Buffer Chromatin IP Elution Buffer Reverse Cross-linking DNA Elution

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