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Epigenetic Inheritance and ChIP in Caenorhabditis elegans

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Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation (Video) | JoVE

JoVE Journal
Genética

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JoVE Journal Genética

Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation

Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation (Video) | JoVE

Epigenetic Inheritance and ChIP in Caenorhabditis elegans

DOI:

10.3791/200310-v

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02:41 min

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May 05, 2023

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Chengyin Li*¹, Phoebe A. W. Bhagoutie*¹, Victor Lao, Arneet L. Saltzman

¹Department of Cell and Systems Biology,University of Toronto

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Keywords: Sonication Formaldehyde-Crosslinked Caenorhabditis Elegans Chromatin Immunoprecipitation Resuspension Buffer Detergents Lysate Centrifugation Immunoprecipitation Anti-H3K9 Trimethylation Anti-histone H3 IgG Protein G Magnetic Beads FA-150 Buffer Chromatin IP Elution Buffer Reverse Cross-linking DNA Elution

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