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Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
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Neurociência
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JoVE Journal Neurociência
Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

DOI:
10.3791/1773-v

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12:23 min

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May 25, 2010

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, , ,
1Molecular Biology of Neural Development,Institut de Recherches Cliniques de Montréal, 2Division of Experimental Medicine and Program in Neuroengineering,McGill University, 3Program in Neuroengineering,McGill University, 4Montreal Neurological Institute, 5Department of Anatomy and Cell Biology,McGill University, 6Department of Biology,McGill University, 7Department of Medicine,Universite de Montreal – University of Montreal

Capítulos

  • 00:00Título
  • 00:45Introduction
  • 01:17Spinal Cord Dissection
  • 05:41Dorsal Spinal Cord Dissection
  • 06:53Commissural Neuron Culture
  • 11:28Representative Cultures And Staining Of Dorsal Spinal Cord Neurons
  • 11:55Conclusion

Summary

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This video demonstrates a method to dissect and culture commissural neurons from E13 rat dorsal spinal cord. Dissociated commissural neurons are useful to study the cellular and molecular mechanisms of axon growth and guidance.

Tags

DissectionCultureCommissural NeuronsEmbryonic Spinal CordAxon GuidanceDorsal Spinal CordStereotyped TrajectoriesGuidance CuesTurning AssaysImmunochemistryBioquímicaE13 RatDissociationCell SuspensionPoly-L-lysine-coated Glass CoverslipsIn Vitro StudiesCellular MechanismsMolecular Mechanisms

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