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激活的B细胞CD40的小鼠模型
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JoVE Journal Imunologia e Infecção
Murine Model of CD40-activation of B cells
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激活的B细胞CD40的小鼠模型

DOI:
10.3791/1734-v

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12:24 min

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March 05, 2010

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, , , ,
1Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine,University Hospital of Cologne

Capítulos

  • 00:00Título
  • 00:14Introduction
  • 02:10Preparation of Murine Splenocytes for CD40 – Stimulation
  • 04:19Preparation of Feeder Cells
  • 07:59CD40 – Stimulation
  • 08:50Subcultivation
  • 11:25Analysis of Murine CD40 – Activated B Cells
  • 11:47Conclusion

Summary

Tadução automática

Tadução automática

在这个视频中，我们展示的CD40从C57BL / 6小鼠的脾细胞，它可以用来作为模型抗原提呈细胞（APC）的研究免疫诱导小鼠的B细胞的活化和扩大的过程。

Tags

CD40-activationB CellsAntigen PresentationInterleukin-4CD40 LigandExpansionPeripheral BloodCD40-B CellsLymph Node Homing MoleculesT CellsAntigensMemory T CellsImmuno-stimulatory Antigen-presenting Cells (APC)Cell-based ImmunotherapyMurine CD40-activated B CellsSplenocytesPurified B CellsC57BL/6 MiceHaplotype H-2bDensity Gradient CentrifugationHeLa CellsRecombinant Murine CD40 Ligand (tmuCD40L HeLa)

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