É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Título
- 00:51Introduction
- 01:18Preparation of the DNA Replication Template
- 03:41Bead Preparation
- 05:28Preparing Functionalized Coverslips
- 08:31Flow Chamber Assembly
- 11:34Single-Molecule Replication Experiment
- 15:41Single-Molecular Replication Data Analysis and Representative Results
- 16:34Conclusion
Tags
EnzymesReplicating DNASingle-molecule DNA Stretching AssayBacteriophage Replication SystemBiotinDigoxigeninFunctionalized Glass CoverslipSmall BeadDNA-bead TethersFlow CellLaminar FlowDrag ForceStretched DNACoverslip SurfaceFlow RateLength MeasurementSingle-stranded DNADouble-stranded DNAReplication ProteinsForkUnwindingPolymerization
