É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Título
- 00:35Introduction
- 01:50Enucleation of Murine Eyes and Dissection of Retinas
- 04:02Dissection of Embryonic, Postnatal and Adult Murine Retinas
- 11:40Murine Organotypic Retinal Wholemount Culture
- 14:10Dissociation of Cultured Retinal Wholemounts
- 19:35Washing of Dissociated Cell Suspensions
- 24:49Conclusion
Este artículo video demuestra el establecimiento de cultivos de retina organotípicos wholemount y un procedimiento citospina para el análisis de los efectos inducidos exógenamente. Organotípicos culturas wholemount imitar la retina<em> In vivo</em> Situación y facilitan significativamente la accesibilidad de las retinas murino de manipulaciones experimentales eludiendo las desventajas de los clásicos modelos animales murinos.
Tags
In Vivo-like Organotypic Murine Retinal Wholemount CultureTargeted Gene AblationsAnimal ModelsRetinal Gene FunctionTransgenic Mouse ModelsConditional Knockout Mouse ModelsPrimary Cell CultureRecombinant FactorsGene OverexpressionSiRNA-mediated Gene KnockdownControlled EnvironmentDissociated Cell CultureEndogenous SignalsExogenously Triggered EffectsPharmacological ManipulationOrganotypic Retinal Wholemount CulturesPhysiological In Vivo SituationNeuronal InteractionsConnections PreservedDissection PeculiaritiesEmbryonic EyesPostnatal EyesAdult Murine EyesNeuronal ApoptosisRetinal Cell Proliferation
