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Immunology and Infection

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JoVE Journal Immunology and Infection
Depletion of Specific Cell Populations by Complement Depletion
 
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Depletion of Specific Cell Populations by Complement Depletion

Article DOI: 10.3791/1487-v • 06:17 min • February 5th, 2010
February 5th, 2010 •
1
1BloodCenter of Wisconsin, Blood Research Institute

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To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.

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Depletion Specific Cell Populations Complement Depletion Immune Cell Purification Real-time PCR Microarray Analysis Fluorescent Activated Cell Sorting (FACS) Magnetic Bead Separation Complement System Plasma Proteins Proteolytic Cascade Membrane-attack Complex Cell Death Classical Pathway IgM Antibodies IgG Antibodies Monoclonal Antibodies (mAb) Antigen-specific Manner Rabbit Complement Incubation Washing
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