JOVE JOURNAL

Bioquímica

Showing 193 - 204 in 759 videos.

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Gerard Castro-Linares, Jeffrey den Haan, Francois Iv, Carla Silva Martins, Aurélie Bertin, Manos Mavrakis, Gijsje H. Koenderink

¹Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology, ²Institut Fresnel, CNRS UMR7249,Aix Marseille Univ, Centrale Marseille, ³Institut Curie, Université PSL,Sorbonne Université

Journal (Biochemistry)

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Lin Cheng*¹, Lantian Su*¹, Xiaowen Tian*¹, Fan Xia, Chang Zhao, Wei Yan, Zhenhua Shao

¹Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital,Sichuan University, ²Department of Neurosurgery, West China Hospital,Sichuan University

Journal (Biochemistry)

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Elizabeth M. Bailey, Emanuel Salazar-Cavazos, Rachel M. Grattan, Michael J. Wester, David J. Schodt, Julian A. Rojo, Keith A. Lidke³, Diane S. Lidke³

¹Department of Pathology,University of New Mexico School of Medicine, ²Department of Physics and Astronomy,University of New Mexico, ³Comprehensive Cancer Center,University of New Mexico Health Sciences Center

Journal (Biochemistry)

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Armand M. G. Jaminon*¹, Nikolas Rapp*¹, Asim C. Akbulut, Robert Dzhanaev, Chris P. Reutelingsperger, Willi Jahnen-Dechent, Leon J. Schurgers³

¹Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, ²Helmholtz Institute for Biomedical Engineering, Biointerface Group,RWTH Aachen University, ³Institute of Experimental Medicine and Systems Biology,RWTH Aachen University

Journal (Biochemistry)

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Joann Phan, Joseph Kapcia III, Cynthia I. Rodriguez, Victoria L. Vogel, Daniel B Cardin, Sage J. B. Dunham, Katrine Whiteson

¹Department of Molecular Biology and Biochemistry,University of California Irvine, ²Department of Ecology and Evolutionary Biology,University of California Irvine, ³Entech Instruments Inc.

Journal (Biochemistry)

Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass

Kenneth J. Olejar*¹, Min Hong*², Sun-Yeop Lee, Tae-Hyung Kwon, Soo-Ung Lee, Chad A. Kinney³, Joon-Hee Han, Sang-Hyuck Park

¹Department of Chemistry,Colorado State University-Pueblo, ²Chuncheon Bioindustry Foundation, ³Institute for Cannabis Research,Colorado State University-Pueblo

Journal (Biochemistry)

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Khanit Sa-ngiamsuntorn, Piyanoot Thongsri, Yongyut Pewkliang, Suparerk Borwornpinyo⁴, Adisak Wongkajornsilp

¹Department of Biochemistry, Faculty of Pharmacy,Mahidol University, ²Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital,Mahidol University, ³Excellent Center for Drug Discovery, Faculty of Science,Mahidol University, ⁴Department of Biotechnology, Faculty of Science,Mahidol University, ⁵Department of Pharmacology, Faculty of Medicine Siriraj Hospital,Mahidol University

Journal (Biochemistry)

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Patrícia M. S. dos Passos*¹, Valentine Spagnol*², Camila R.S.T.B de Correia, Felipe R. Teixeira²

¹Department of Genetic and Evolution,Federal University of São Carlos, ²Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto,University of São Paulo

Journal (Biochemistry)

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Hugo Wioland, Foad Ghasemi, Jahnavi Chikireddy, Guillaume Romet-Lemonne, Antoine Jégou

¹Université Paris Cité, CNRS,Institut Jacques Monod

Journal (Biochemistry)

Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry

Marcos A. Formiga-Jr, Juliana Camacho-Pereira

¹Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde,Universidade Federal do Rio de Janeiro

Journal (Biochemistry)

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Dylan Nicholas Tabang, Danqing Wang, Lingjun Li²

¹Department of Chemistry,University of Wisconsin-Madison, ²School of Pharmacy,University of Wisconsin-Madison

Journal (Biochemistry)

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Yazgan Tuna, Amer Al-Hiyasat, Jonathon Howard²

¹Department of Molecular Biophysics & Biochemistry,Yale University, ²Department of Physics,Yale University

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