परख के लिए तरीके ड्रोसोफिला व्यवहार
Summary
ड्रोसोफिला मेलानोगास्टर एक आनुवंशिक और behaviorally विनयशील मॉडल प्रणाली है कि एक एक सदी से भी अधिक के लिए कई महत्वपूर्ण जैविक प्रक्रियाओं की आणविक और सेलुलर आधार को समझने के लिए इस्तेमाल किया गया है है ड्रोसोफिला अच्छी तरह से किया गया है मक्खी व्यवहार के आनुवंशिक आधार में अंतर्दृष्टि लाभ का फायदा उठाया है.
Abstract
Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular disease and various neurological diseases1. We have optimized simple and robust behavioral assays for determining larval locomotion, adult climbing ability (RING assay), and courtship behaviors of Drosophila. These behavioral assays are widely applicable for studying the role of genetic and environmental factors on fly behavior. Larval crawling ability can be reliably used for determining early stage changes in the crawling abilities of Drosophila larvae and also for examining effect of drugs or human disease genes (in transgenic flies) on their locomotion. The larval crawling assay becomes more applicable if expression or abolition of a gene causes lethality in pupal or adult stages, as these flies do not survive to adulthood where they otherwise could be assessed. This basic assay can also be used in conjunction with bright light or stress to examine additional behavioral responses in Drosophila larvae. Courtship behavior has been widely used to investigate genetic basis of sexual behavior, and can also be used to examine activity and coordination, as well as learning and memory. Drosophila courtship behavior involves the exchange of various sensory stimuli including visual, auditory, and chemosensory signals between males and females that lead to a complex series of well characterized motor behaviors culminating in successful copulation. Traditional adult climbing assays (negative geotaxis) are tedious, labor intensive, and time consuming, with significant variation between different trials2-4. The rapid iterative negative geotaxis (RING) assay5 has many advantages over more widely employed protocols, providing a reproducible, sensitive, and high throughput approach to quantify adult locomotor and negative geotaxis behaviors. In the RING assay, several genotypes or drug treatments can be tested simultaneously using large number of animals, with the high-throughput approach making it more amenable for screening experiments.
Protocol
Discussion
ड्रोसोफिला व्यवहार कस आनुवांशिक और पर्यावरणीय कारकों द्वारा नियंत्रित किया जाता है. हम, और दूसरों को, पहले यहाँ वर्णित assays के व्यवहार और मानव neurodegenerative ड्रोसोफिला 5-19 में मॉडलिंग की बीमारियों के…
Declarações
The authors have nothing to disclose.
Acknowledgements
हम के लार्वा रेंगने डेटा पैदा करने के लिए आस्था Maltare धन्यवाद देना चाहूंगा. हम डॉ. निकोलस लांसन जूनियर पांडुलिपि पर अपनी टिप्पणी देने के लिए धन्यवाद देना चाहूंगा. इस काम के जॉन्स हॉपकिन्स पर रॉबर्ट ए एल एस के लिए पैकार्ड केंद्र (UBP के लिए) और Amyotrophic पार्श्व स्केलेरोसिस एसोसिएशन (UBP) द्वारा समर्थित किया गया था, और मानसिक स्वास्थ्य के राष्ट्रीय संस्थान (CDN) से R01MH083689.
Materials
| Name of the reagent | Company | Catalogue number |
| Sucrose | Fisher Scientific | S5-500 |
| Agarose | Invitrogen | 16500-500 |
| 6 oz Drosophila bottle | Genesee Scientific | 32-130 |
| Paint Brush (#1) | Ted Pella,Inc. | 11859 |
| Fly food components | ||
| Cornmeal | Fisher Scientific | NC9109741 |
| Agar | Genesee Scientific | 66-104 |
| Molasses | Fisher Scientific | NC9349176 |
| Propionic acid | Acros | 14930-0010 |
| Tegosept | Apex | 20-258 |
| Ethanol | Fisher Scientific | BP2818-4 |
| Yeast | Genesee Scientific | 62-107 |
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