Assessing the Antimicrobial Potential of Bacteriocin-Producing Murine Fecal Bacterial Isolates
Published: February 29, 2024
Abstract
Source: Bäuerl, C., et al. A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice. J. Vis. Exp. (2017).
This video demonstrates the detection of bacteriocin-producing lactic acid bacteria derived from mouse fecal matter. The assay determines the antimicrobial activity of bacteriocin on specific bacterial strains.
Protocol
1. LAB Counting and Bacteriocin Activity Weigh one fecal pellet of each sample in a 1.5 mL tube and add an adequate volume of ice-cold 0.9% NaCl to achieve a 10% (w/v) solution. Use sterile micro pestles for 1.5 mL tubes to homogenize the fecal suspension and prepare ten-fold serial dilutions in ice-cold 0.9% NaCl. Count the total number of LAB cells. Transfer diluted cells (100 µL) to 4 mL of prewarmed Man Rogosa Sharpe (MRS) soft agar (50 °C, 0.8% agar). Mix …
Representative Results
Figure 1: Detection of Bacteriocin Production in LAB Recovered from Feces by a Three-layer Protocol (as described in the procedure)
Declarações
The authors have nothing to disclose.
Materials
| Plastic Petri dish | Thermo Scientific | 101VR20 | |
| Brain-Heart-Infusion broth | Conda | 1400 | |
| European Bacteriological Agar | Pronadisa | 1800 | |
| Agarose D1 Low EEO | Pronadisa | 8010 | |
| 1x TAE buffer | Thermo Scientific | 15558042 | |
| MRS broth | Difco | 288130 | |
| PBS tablets | Sigma | P4417-100TAB | |
| Scale | Mettler Toledo | PB602-S |
Tags
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Assessing the Antimicrobial Potential of Bacteriocin-Producing Murine Fecal Bacterial Isolates. J. Vis. Exp. (Pending Publication), e21973, doi: (2024).