A Technique to Generate an Adjuvant-Induced Arthritis Mouse Model
Published: September 29, 2023
Abstract
Source: Lu, Q., et al. Detecting Migration and Infiltration of Neutrophils in Mice. J. Vis. Exp. (2020).
This video demonstrates a method to generate adjuvant-induced arthritis in a murine model. Complete Freund's adjuvant (CFA) is injected in the mouse ankle joint and periarticular areas. CFA activates the immune system, leading to neutrophil infiltration and exaggerated inflammation.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Induction of the adjuvant-induced arthritis (AA) mouse model Suspend complete Freund's adjuvant (CFA) by vortexing at least 5 s, then draw 100 µL of suspension into an insulin injector. NOTE: We recommend using a completely new CFA in experiments to ensure sterility. Anesthetiz…
Representative Results
Figure 1: Representative results of the adjuvant-induced arthritis (AA) mouse model. (A) The ankle joint diameter was measured using a pocket thickness gauge. (B) Joint swelling was assessed based on the ankle joint diameter (n = 7). (C) Pictures of each arthritis score. (D) The severity of arthritis was graded on a scale of 0…
Declarações
The authors have nothing to disclose.
Materials
| 15 mL Conical Polypropylene Centrifuge Tube | Falcon | 14-959-53A | |
| 23 G x 1 1/4" Needle | BD | 305120 | |
| 26 G x 3/8" Needle | BD | 305110 | |
| 30 G x 1/2" Needle | BD | 305106 | |
| Complete Freund's Adjuvant, 10 mg/ml | sigma | 1002036152 | |
| Eppendorf Microtubes, 1.5 mL | Sigma | Z606340 | |
| Dial Thickness Gauge | Mitutoyo | 7301 | |
| Shu Rui U40 Disposable Sterile Insulin Injection Needle 1 mL | BD | 328421 |
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A Technique to Generate an Adjuvant-Induced Arthritis Mouse Model. J. Vis. Exp. (Pending Publication), e21709, doi: (2023).