Mosquito Feeding Assay: A Method to Evaluate Test Compound Effects on Mortality and Fecundity of Adult Female Mosquitoes
Published: April 30, 2023
Abstract
Source: Brito-Sierra, C. A. et al., Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes. J. Vis. Exp. (2019).
This video demonstrates a blood-feeding assay to evaluate the mortality and fecundity of test compounds with novel modes of action on female mosquitoes. This technique helps in screening new classes of insecticides to kill the resistant population of mosquitoes and control disease spread.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Adult Blood-Feeding Assay (Single Point Dose or Dose-Response Assay) Collect approximately 150, 4-5-day old adult female mosquitoes and transfer to a separate cage. Repeat to obtain 6 cages if performing a dose-response assay. Remove the source of sugar 1-24 h prior to the feeding assay. Prepare a stock solution of chemistry as …
Declarações
The authors have nothing to disclose.
Materials
| Insecticide susceptible mosquito strains | Malaria Research and Reagent Reference Repository | https://www.beiresources.org/ MR4Home.aspx; Recommended strains: Aedes aegypti Liverpool (LVP) strain, Anopheles gambiae Kisumu (KISUMU1) strain and Culex quinquefasciatus Johannesburg (JHB) strain | |
| Bifenthrin | Sigma-Aldrich | CAS: 51529-01-2 | Synthetic pyrethorid used as positive control |
| Defibrinated rabbit blood | Hemostat | DRB0100 | Blood source for adapted mosquitoes to artificial feeding |
| Hemotek membrane feeding system | Hemotek Ltd | Serial no. 1303 | Used for feeding mosquitoes |
| Transfer pipettes | Fisher Scientific | 13-711-7M U | |
| Stereo microscope | Olympus | SZ6045 | Used to score larval assays and perform micro-applications |
| Tweezers (fine) | Fontax | Used to handle adult mosquitoes | |
| Paper cups | Dixie Consumer Products LLC. | PF15675/13D | Used to keep adult mosquitoes in adult topical assays |
| Advantage rubber bands | Alliance Rubber Co. | Used to seal the paper cups |
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Mosquito Feeding Assay: A Method to Evaluate Test Compound Effects on Mortality and Fecundity of Adult Female Mosquitoes. J. Vis. Exp. (Pending Publication), e21171, doi: (2023).