Harvesting Articular Cartilage From Joints of an Equine Donor: A Surgical Procedure to Isolate Vascular Connective Tissue From Cadaveric Joints of an Equine Donor
Published: April 30, 2023
Abstract
Source: Benders, K. E. M. et al. Fabrication of Decellularized Cartilage-derived Matrix Scaffolds. J. Vis. Exp. (2019)
In this video, we demonstrate the extraction of articular cartilage from an equine cadaveric joint. The isolated articular cartilage can be used for further downstream analysis.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board 1. Harvesting of Articular Cartilage from Donor (Cadaveric) Joints Ahead of the harvesting step, prepare 1 L of cartilage washing solution, consisting of sterile phosphate-buffered saline (PBS), supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin, and 1% (v/v) Amphotericin B. Wear gloves and a lab coat during the en…
Representative Results
Figure 1: Equine knee after removing full-thickness cartilage. The cartilage is removed from the condyles using a scalpel until the calcified cartilage layer that cannot be cut using a scalpel is reached. Figure 2: Sequ…
Declarações
The authors have nothing to disclose.
Materials
| Cadaveric joint | This can be obtained as rest material from the local butcher or veterinary center. | ||
| Sterile phosphate-buffered saline (PBS) | |||
| Penicillin-Streptomycin | Gibco | 15140 | |
| Amphotericin B | Thermo Fischer Scientific | 15290026 | |
| Liquid nitrogen | |||
| Freeze-dryer | SALMENKIPP | ALPHA 1-2 LD plus | |
| Centrifuge (for 50 mL tubes) | Eppendorf | 5810R | |
| Scalpel | |||
| Scalpel holder | |||
| Small laddle |
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Harvesting Articular Cartilage From Joints of an Equine Donor: A Surgical Procedure to Isolate Vascular Connective Tissue From Cadaveric Joints of an Equine Donor. J. Vis. Exp. (Pending Publication), e20922, doi: (2023).