Ultrasound-Guided Concanavalin A Injection Into Inferior Lacrimal Gland: A Technique to Inoculate Inferior Lacrimal Gland With Concanavalin A to Induce Dry Eye Disease in Rabbit Model
Published: April 30, 2023
Abstract
Source: Honkanen, R. A. et al. A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies. J. Vis. Exp. (2020)
In this video, we demonstrate the inoculation of Concanavalin A, a lymphocyte mitogen, into the inferior lacrimal gland of the eye of a rabbit to induce dry eye disease. Ultrasound or sonogram images are used to guide the precise location of the injection site and also to confirm the success of inoculation.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Animals and Housing Acquire New Zealand White (NZW) rabbits weighing 2-3 kg. House the rabbits singly in cages with strict temperature (65 ± 5 °F) and humidity (45 ± 5%) control. Lighting should have a 12 h on/off cycle. Provide unlimited access to water and standard rabbit chow. Eliminate dietary enri…
Representative Results
Figure 1: Preparation of rabbit for concanavalin A injections. (A) Small shears are used to remove fur, allowing easier visualization of landmarks to identify the orbital superior lacrimal gland. (B) Nair is used to remove hair that remains after shearing. <str…
Declarações
The authors have nothing to disclose.
Materials
| Rabbit, New Zealand White | Charles River Labs, Waltham, MA | Research animals, 2-3 kg | |
| Aceproinj (acepromazine) | Henry Schein Animal Health, Dublin, OH | NDC11695-0079-8 | 0.1ml/kg subcutaneously injection for rabbit sedation |
| Ultrasound probe | VisualSonics Toronto, Ont | MX 550 S | Untrasonography-guide ConA injection for inferior lacrimal gland |
| Caliper | Bausch and Lomb (Storz), Bridgewater, NJ | E-2404 | Caliper used to measure length of needle during ConA injection |
| Concanavalin A | Sigma, St. Louis, MO | C2010 | Make 5mg/ml in PBS for injection into rabbit lacrimal glands |
| Isoflurane | Henry Schein, Melville, NY | 29405 | |
| Anesthesia vaporizer | VetEquip, Pleasanton, CA | Item #911103 | |
| 26-gauge needles (5/8) | Becton Dickinson and Company, Franklin Lakes, NJ | 305115 | Needles for injecting ConA into the lacrimal glands |
| 27-gauge needles (5/8) | Becton Dickinson and Company, Franklin Lakes, NJ | 305921 | Needles for injecting ConA into the lacrimal glands |
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Ultrasound-Guided Concanavalin A Injection Into Inferior Lacrimal Gland: A Technique to Inoculate Inferior Lacrimal Gland With Concanavalin A to Induce Dry Eye Disease in Rabbit Model. J. Vis. Exp. (Pending Publication), e20911, doi: (2023).