Laser Doppler Needle Probe Insertion: A Technique for Real-time Quantification of Blood Flow within the Spinal Cord in a Porcine Model

Published: April 30, 2023

Abstract

Source: Behem, C. R. et al., Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion. J. Vis. Exp. (2020).

This video demonstrates the placement of a laser Doppler needle probe in the spinal cord of a porcine model. This helps in the continuous quantification of blood supply to the spinal cord, an important assessment in spinal cord injury.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Probe placement Place the anesthetized and ventilated pig in right lateral position and flex the animal's back to widen the space between the vertebrae. Surgically expose the paravertebral area for the preparation of spinous processes and vertebral arches (Figure 1A). Place a vascular 14 G periph…

Representative Results

Figure 1: Placement of laser/Doppler needle probe in the spinal cord. (A) Surgical exposure of vertebral structures. (B) Puncture of the spinal cord using a vein catheter. (C) Insertion of the needle probe after removal of the inlay needle. (D) Fixation of the needle probe.

Declarações

The authors have nothing to disclose.

Materials

Infinity Delta Dräger Medical, Lübeck, Germany Basic Monitoring Hardware
Infinity Hemo Dräger Medical, Lübeck, Germany Basic Pressure Monitoring and Pulmonary Thermodilution Hardware
LabChart Pro ADInstruments Ltd., Oxford, UK v8.1.16 Synchronic Laser-Doppler, Blood Pressure, ECG and Blood-Flow Aquisition Software
moor VMS LDF moor Instruments, Devon, UK Designated Laser-Doppler Hardware
moor VMS Research Software moor Instruments, Devon, UK Designated Laser-Doppler Software
PiCCO 2, Science Version Getinge AB, Göteborg, Sweden v. 6.0 Blood Pressure and Transcardiopulmonary Monitoring Hard- and Software
PowerLab ADInstruments Ltd., Oxford, UK PL 3516 Synchronic Laser-Doppler, Blood Pressure, ECG and Blood-Flow Aquisition Hardware
QuadBridgeAmp ADInstruments Ltd., Oxford, UK FE 224 Four Channel Bridge Amplifier for Laser-Doppler and Invasive Blood Pressure Aquisition
Twinwarm Warming System Moeck & Moeck GmbH, Hamburg, Germany 12TW921DE Warming System
VP 3 Probe, 8mm length (individually manufactured) moor Instruments, Devon, UK Laser-Doppler Probe
Zeus Dräger Medical, Lübeck, Germany Anesthesia Machine

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Laser Doppler Needle Probe Insertion: A Technique for Real-time Quantification of Blood Flow within the Spinal Cord in a Porcine Model. J. Vis. Exp. (Pending Publication), e20840, doi: (2023).

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