Laser Doppler Needle Probe Insertion: A Technique for Real-time Quantification of Blood Flow within the Spinal Cord in a Porcine Model
Published: April 30, 2023
Abstract
Source: Behem, C. R. et al., Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion. J. Vis. Exp. (2020).
This video demonstrates the placement of a laser Doppler needle probe in the spinal cord of a porcine model. This helps in the continuous quantification of blood supply to the spinal cord, an important assessment in spinal cord injury.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Probe placement Place the anesthetized and ventilated pig in right lateral position and flex the animal's back to widen the space between the vertebrae. Surgically expose the paravertebral area for the preparation of spinous processes and vertebral arches (Figure 1A). Place a vascular 14 G periph…
Representative Results
Figure 1: Placement of laser/Doppler needle probe in the spinal cord. (A) Surgical exposure of vertebral structures. (B) Puncture of the spinal cord using a vein catheter. (C) Insertion of the needle probe after removal of the inlay needle. (D) Fixation of the needle probe.
Declarações
The authors have nothing to disclose.
Materials
| Infinity Delta | Dräger Medical, Lübeck, Germany | Basic Monitoring Hardware | |
| Infinity Hemo | Dräger Medical, Lübeck, Germany | Basic Pressure Monitoring and Pulmonary Thermodilution Hardware | |
| LabChart Pro | ADInstruments Ltd., Oxford, UK | v8.1.16 | Synchronic Laser-Doppler, Blood Pressure, ECG and Blood-Flow Aquisition Software |
| moor VMS LDF | moor Instruments, Devon, UK | Designated Laser-Doppler Hardware | |
| moor VMS Research Software | moor Instruments, Devon, UK | Designated Laser-Doppler Software | |
| PiCCO 2, Science Version | Getinge AB, Göteborg, Sweden | v. 6.0 | Blood Pressure and Transcardiopulmonary Monitoring Hard- and Software |
| PowerLab | ADInstruments Ltd., Oxford, UK | PL 3516 | Synchronic Laser-Doppler, Blood Pressure, ECG and Blood-Flow Aquisition Hardware |
| QuadBridgeAmp | ADInstruments Ltd., Oxford, UK | FE 224 | Four Channel Bridge Amplifier for Laser-Doppler and Invasive Blood Pressure Aquisition |
| Twinwarm Warming System | Moeck & Moeck GmbH, Hamburg, Germany | 12TW921DE | Warming System |
| VP 3 Probe, 8mm length (individually manufactured) | moor Instruments, Devon, UK | Laser-Doppler Probe | |
| Zeus | Dräger Medical, Lübeck, Germany | Anesthesia Machine |
Tags
Citar este artigo
Laser Doppler Needle Probe Insertion: A Technique for Real-time Quantification of Blood Flow within the Spinal Cord in a Porcine Model. J. Vis. Exp. (Pending Publication), e20840, doi: (2023).