Ex Situ Heart Perfusion: A Technique to Preserve Isolated Porcine Heart Function
Published: April 30, 2023
Abstract
Source: Hatami, S. et. al., Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism. J. Vis. Exp. (2019).
This video describes an ex situ technique for preserving an isolated porcine heart by perfusing it with an oxygenated nutrient-rich perfusate solution. The technique allows the comprehensive evaluation of cardiac performance under physiological conditions.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Pre-surgical Preparations Place the organ chamber properly on the apparatus cart and install the silicon support membrane inside the organ chamber. The aorta (Ao), pulmonary artery (PA) and left atrium (LA) connection points can be seen in Figure 1. Install the ex situ heart perfusion (ESHP) tubing network (re…
Representative Results
Figure 1: The silicone support membrane for the heart. Support membrane pictured with integrated aortic cannula (A), left atrial cannula (B), and pulmonary artery cannula (C). Figure 2: The ESHP circuit. (A) Schematic figure of the ES…
Declarações
The authors have nothing to disclose.
Materials
| Debakey-Metzenbaum dissecting scissors | Pilling | 342202 | |
| MAYO dissecting scissors | Pilling | 460420 | |
| THUMB forceps | Pilling | 465165 | |
| Debakey straight vascular tissue forceps | Pilling | 351808 | |
| CUSHING Gutschdressing forceps | Pilling | 466200 | |
| JOHNSON needle holder | Pilling | 510312 | |
| DERF needle holder | Pilling | 443120 | |
| Sternal saw | Stryker | 6207 | |
| Sternal retractor | Pilling | 341162 | |
| Vorse tubing clamp | Pilling | 351377 | |
| MORRIS ascending aorta clamp | Pilling | 353617 | |
| Surgical snare (tourniquet) set | Medtronic | CVR79013 | |
| 2-0 SILK black 12" x 18" strands | ETHICON | A185H | |
| 3-0 PROLENE blue 18" PS-2 cutting | ETHICON | 8687H | |
| Biomedicus pump drive (modified) | Medtronic | 540 | Modified to allow remote electronic control of pump speed |
| Biomedicus pump | Maquet | BPX-80 | |
| Membrane oxigenator D 905 | SORIN GROUP | 50513 | |
| Tubing flow module | Transonic | Ts410 | |
| PXL clamp-on flow sensor | Transonic | ME9PXL-BL37SF | |
| TruWave pressure transducer | Edwards | VSYPX272 | |
| Intercept tubing 3/8" x 3/32" xX 6' | Medtronic | 3506 | |
| Intercept tubing 1/4" x 1/16" x 8' | Medtronic | 3108 | |
| Heated/Refrigerated Bath Circulator | Grant | TX-150 | |
| ABL 800 FLEX Blood Gas Analyzer | Radiometer | 989-963 | |
| DLP cardioplegia cannula | Medtronics | 20613994495406 | Aortic root cannula |
| 5F Ventriculr straight pigtail cathter | CORDIS | 534550S | |
| 5F AVANTI+ Sheath Introducer | CORDIS | 504605A | |
| Emerald Amplatz Guidewire | CORDIS | 502571A | |
| Dual chamber pace maker | Medtronic | 5388 | |
| Defibrilltor | CodeMaster | M1722B | |
| Infusion pump | Baxter | AS50 | |
| Surgical electrocautery device | Kls Martin | ME411 | |
| Gas mixer | SECHRIST | 3500 CP-G | |
| Medical oxygen tank | Praxair | 2014408 | |
| Carbon dioxide tank | Praxair | 5823115 | |
| Bovine serum albumin | MP biomedicals | 218057791 |
Tags
Citar este artigo
Ex Situ Heart Perfusion: A Technique to Preserve Isolated Porcine Heart Function. J. Vis. Exp. (Pending Publication), e20839, doi: (2023).