Locating the Cisterna Magna in a Pig Model: A Surgical Method to Access the Cisterna Magna in a Pig Model for Direct Cannulation
Published: April 30, 2023
Abstract
Source: Bèchet, N. B., et al. Direct Cannula Implantation in the Cisterna Magna of Pigs. J. Vis. Exp. (2021).
In this video, we describe a method to access the cisterna magna in a pig model. The cisterna magna is a preferred site for studies concerning cerebrospinal fluid.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Animal Preparation Sedate a pig by intramuscular (i.m.) injection of tiletamine (3.75 mg/kg) and zolazepam (3.75 mg/kg) and dexmedetomidine (37.5 μg/kg). Wait for it to become unconscious. Prepare an intravenous line by inserting a 20 G cannula into the ear vein. NOTE: Make sure the cannula is in t…
Representative Results
Figure 1. Cisterna magna cannulation in pigs. (A) Pig prepped prior to the start of the surgery and marked where dermal incisions will be performed starting from the occipital crest (OC) then posterior to thoracic vertebrae (TV) and lateral to each ear base (EB). (B) Head in the relaxed position with the trapezius, semispinalis capitus biventer and semi…
Declarações
The authors have nothing to disclose.
Materials
| Anatomical forceps | NA | NA | |
| Marker pen | NA | NA | |
| No. 21 Scalpel blade | Agnthos | BB521 | |
| No. 4 Scalpel handle | Agnthos | 10004-13 | |
| Self-retaining retractors | NA | NA | |
| Surgical forceps | NA | NA |
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Locating the Cisterna Magna in a Pig Model: A Surgical Method to Access the Cisterna Magna in a Pig Model for Direct Cannulation. J. Vis. Exp. (Pending Publication), e20821, doi: (2023).