Continuous Drug Infusion System in Mouse Model: A Surgical Procedure to Implant Micro-osmotic Pump Infusion System in the Mouse Brain for Continuous Drug Delivery
Published: April 30, 2023
Abstract
Source: Ajoy, R., Chou, S. Y. Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain. J. Vis. Exp. (2018)
In this video, we demonstrate the assembly of a micro-osmotic pump brain infusion system followed by its surgical implantation in the mouse skull. This system enables continuous delivery of potential drugs and facilitates the study of their effects on the brain and brain tissue.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Micro-Osmotic Pump Infusion Systems NOTE: Prepare the pump, artificial cerebrospinal fluid (aCSF) buffer, and drug (Met-CCL5/RANTES protein solution (10 ng/mL in aCSF)) under sterile conditions using buffers filtered with 0.2 µm filters and conduct all the procedures un…
Representative Results
Figure 1. Osmotic pump preparation and implantation surgical procedure in mouse. (A) The brain infusion kit and pump preparation perfused with drug solution. Red arrows indicate the catheter tubes filled with liquid. (B) Fix and mount the mouse head onto the stereotactic apparatus. (C) Separate the outermost layer of the skin from the subcutaneous skin for the im…
Declarações
The authors have nothing to disclose.
Materials
| Vetbond Tissue Adhesive | 3M | #1049SB | The glue used to seal the lesion site on the mouse head |
| LOCTITE 454 instant adhesive | Durect Corporation | #8670 | The glue used to fix the needle on the mouse skull |
| Alzet Micro- Osmotic Pump | Durect Corporation | #9922 | 0.11 μl per hour, 28 days |
| Brain infusion system | Durect Corporation | #8851 | 1-3 mm, used to perfuse the drug in to the mice brain |
| MIO NE116 CONTROL UNIT (nail drill) | Mio System | #E235-015 | To drill a hole in the skull of the mouse |
| aCSF formula | 119 mM NaCl 26.2 mM NaHCO3 2.5 mM KCl 1 mM NaH2PO4 1.3 mM MgCl2 10 mM glucose | Filter sterilize with a 0.22 μm filter apparatus, and store at 4°C. aCSF is stable for 3-4 weeks |
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Continuous Drug Infusion System in Mouse Model: A Surgical Procedure to Implant Micro-osmotic Pump Infusion System in the Mouse Brain for Continuous Drug Delivery. J. Vis. Exp. (Pending Publication), e20792, doi: (2023).