Modeling Photothrombotic Stroke in Mouse Model: A Laser Illumination Technique Through Mouse Skull Following Photosensitive Dye Administration to Induce Photothrombosis
Published: April 30, 2023
Abstract
Source: Llovera, G. et al. Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis. J. Vis. Exp. (2021)
This video demonstrates the procedure to generate photothrombotic stroke in a mouse model by laser illumination of an intact skull following administration of photosensitive dye.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of the animal Inject analgesia 30 min before surgery (4 mg/kg Carprofen and 0.1 mg/kg Buprenorphine). Record the mouse body weight to adjust the dose of Rose Bengal to be injected (10 µL/g i.e., 100 µg/g). Place the mouse in the induction chamber with an isoflurane flow rate of …
Declarações
The authors have nothing to disclose.
Materials
| 561 nm wavelenght laser | Solna | Cobolt HS-03 | |
| C57Bl/6J mice | Charles River | 664 | |
| Collimeter | Thorlabs | F240APC-A | |
| Cotons | NOBA Verbondmitel Danz | 974116 | |
| Ethanol 70% | CLN Chemikalien Laborbedorf | 521005 | |
| Fine Scissors | FST | 15000-00 | |
| Forceps | FST | 11616-15 | |
| Heating blanket | FHC DC Temperature Controller | 40-90-8D | |
| Isoflurane | Abbot | B506 | |
| Protective glasses | Laser 2000 | NIR-ZS2-38 | |
| Rose Bengal | Sigma Aldrich | 198250-5G | |
| Saline solution | Braun | 131321 | |
| Stereotactic frame | Stoelting | 51500U | |
| Anesthesia system for isoflurane | Drager | ||
| Phosphate Buffered Saline PH: 7.4 | Apotheke Innestadt Uni Munchen | P32799 |
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Modeling Photothrombotic Stroke in Mouse Model: A Laser Illumination Technique Through Mouse Skull Following Photosensitive Dye Administration to Induce Photothrombosis. J. Vis. Exp. (Pending Publication), e20789, doi: (2023).