Methylene Blue Dye Injection in Mouse Embryonic Urinary Tract: A Method To Assess the Congenital Obstruction in the Urinary Tract
Published: April 30, 2023
Abstract
Source: Yun, K. Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection. J. Vis. Exp. (2017)
In this video, we administer methylene blue dye injection into the urinary tract of a perinatal embryonic mouse to assess congenital anomalies related to urinary tract obstruction.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board 1. Preparation of Methylene Blue Dye Solution Measure 0.1 g methylene blue powder. Dissolve the methylene blue in 10 mL normal saline or 1x phosphate-buffered saline (PBS) completely by vortexing. Filter the 10 mg/mL methylene blue solution with a syringe filter (membrane pore size 0.45µm) t…
Representative Results
Figure 1. Examples of methylene blue dye injection into a normal urinary system. (A) A typical image of the urinary system from a perinatal embryo (E19.5) after removing excess tissue. Two kidneys, two ureters, and a bladder are seen with reproductive organs, here testes (B) An example of dye injection into a normal kidney. An injection point into t…
Declarações
The authors have nothing to disclose.
Materials
| Methylene blue | Sigma-Aldrich | M9140 | |
| Quality Biological Inc. NORMAL SALINE – 500ML | Fisherscientific | 50-983-204 | |
| 3 mL disposable syringe with BD Luer-Lok tip | BD | 309657 | |
| Acrodisc Syringe Filters with Supor Membrane | Pall corporation | 4614 | |
| Exel Scalp Vein (Butterfly) Sets; 27G x 3/4" 12" | EXELINT | 26709 | |
| Delicate Operating Scissors | Roboz Surgical Instrument Co. | RS-6702 | |
| Micro Dissecting Forceps | Roboz Surgical Instrument Co. | RS-5135 | |
| Dumont Tweezers; Pattern #5 | Roboz Surgical Instrument Co. | RS-5045 | |
| BD PrecisionGlide Needles 30G x 1/2" | BD | 305106 |
Tags
Citar este artigo
Methylene Blue Dye Injection in Mouse Embryonic Urinary Tract: A Method To Assess the Congenital Obstruction in the Urinary Tract. J. Vis. Exp. (Pending Publication), e20755, doi: (2023).