Fluorescence-guided Tumor Resection and MSC-seeded Scaffold Implantation in Murine Model: A Surgical Procedure to Deliver Therapeutic Mesenchymal Stem Cells into Brain Tumor Resection Cavity in Mouse Model
Published: April 30, 2023
Abstract
Source: Sheets, K. T. et al. Image-Guided Resection of Glioblastoma and Intracranial Implantation of Therapeutic Stem Cell-seeded Scaffolds. J. Vis. Exp. (2018)
This video presents the surgical procedure for image-guided tumor resection and implantation of a bio-scaffold seeded with genetically engineered mesenchymal stem cells or MSCs expressing tumor necrosis factor-related apoptosis-inducing ligand or TRAIL. These therapeutic MSCs have the potential in treatment of brain tumors.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Fluorescence-guided Resection and Scaffold Implantation NOTE: Sterilize all tools prior to initializing surgery. Place the stereotaxic frame on the stage of a fluorescence dissecting stereomicroscope. Anesthetize the mouse under inhaled isoflurane in an induction ch…
Declarações
The authors have nothing to disclose.
Materials
| Just for mouse stereotaxic instrument | Stoelting | 51730 | Maintains steady head positioning during surgery |
| Fluorescence dissecting stereomicroscope | Leica | M165 FC | Allows real-time imaging of tumor during resection |
| Vetbond tissue adhesive | 3M | 1469 | Sugical glue to close skin wound |
| Artificial tears | Akorn | 664268 | Prevents eyes from drying during surgery |
| Webcol alcohol preps | Covidien | 6818 | Sterilize incision site |
| Betadine surgical scrub | Purdue Fredick Company | 6761815117 | Sterilize incision site |
| Cotton-tipped applicators | Fisherbrand | 23-400-115 | Surgery tool |
| E-vac aspirating system | Argos | EV310 | Vacuum pump used to resect tumor |
| Fibrinogen and thrombin extracted from as-received TISSEEL | Baxter | To temporarily secure the scaffold in the resection cavity |
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Fluorescence-guided Tumor Resection and MSC-seeded Scaffold Implantation in Murine Model: A Surgical Procedure to Deliver Therapeutic Mesenchymal Stem Cells into Brain Tumor Resection Cavity in Mouse Model. J. Vis. Exp. (Pending Publication), e20675, doi: (2023).