Streptozocin Treatment: A Zebrafish Model of Type 1 Diabetes Mellitus
Published: April 30, 2023
Abstract
Source: Intine R. V., et. al. A Zebrafish Model of Diabetes Mellitus and Metabolic Memory. J. Vis. Exp. (2013).
This video describes the technique of creating a zebrafish model for Type 1 diabetes mellitus with the help of the drug Streptozocin, to study the pathophysiology of diabetic complications and metabolic memory.
Protocol
1. Generation of Zebrafish with Diabetes mellitus, DM Fish Prepare both recovery and anesthetic water tanks. Recovery water is normal fish water. For anesthetic water add sufficient 2-phenoxyethanol so that a 1:1,000 dilution in normal fish water is achieved. Prepare a 0.3% solution (in a fume hood) of streptozocin (STZ) by adding 6 mg of STZ to 2 ml of 0.09% sodium chloride and immediately place the solution on ice . This will provide enough injection solution for the injection of approximately 20 …
Materials
| Streptozocin | Sigma Aldrich | S0130 | |
| 2 phenoxyethanol | Sigma Aldrich | P1126 | |
| Scalpel (size 10) | Fisher Scientific | 089275A | |
| Petri Dishes | Fisher Scientific | 08-757-13 | |
| ½ cc syringe, with 27 1/2 gauge needle | Fisher Scientific | 305620 | |
| Sodium Chloride | Sigma Aldrich | S3014 | |
| Dissecting Microscope | Nikon | TMZ-1500 | Any dissecting microscope is fine. |
Citar este artigo
Streptozocin Treatment: A Zebrafish Model of Type 1 Diabetes Mellitus. J. Vis. Exp. (Pending Publication), e20203, doi: (2023).