DNA Extraction from Tail Clip: A Method Used in Zebrafish Genotyping
Published: April 30, 2023
Abstract
Source: Xing L., et al. Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis. J. Vis. Exp. (2014).
This article describes DNA extraction from zebrafish tail clip. The protocol demonstrates a rapid and efficient method to analyze zebrafish genotypes.
Protocol
1. DNA Preparation Prepare DNA lysis buffer: 50 mM KCl, 10 mM Tris-HCl pH 8.3, 0.3% Tween 20, 0.3% NP40. Add fresh Proteinase K to a final concentration of 1 mg/ml on the day of use. Tissue collection: For adult fish fin clip: Anesthetize fish: Place the fish in 0.004% MS-222 (tricaine) solution. Wait until gill movement slows. Put fish on a stack of 5-10 Kimwipes and cut a small piece of the tail fin, about 2-3 mm, with a sterile razor blade. Quickl…
Materials
| 100 Reaction LightScanner Master Mix | BioFire | HRLS-ASY-0002 | www.biofiredx.com |
| Tricaine | |||
| Paraformaldehyde |
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DNA Extraction from Tail Clip: A Method Used in Zebrafish Genotyping. J. Vis. Exp. (Pending Publication), e20184, doi: (2023).