Escape Response Assay: A Method to Study the Response of Zebrafish Larva to Touch Stimuli

Published: April 30, 2023

Abstract

Source: Sztal, T. E., et al. Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in ZebrafishJ. Vis. Exp. (2016)

This video describes the touch-evoke response assay, which measures the response of the zebrafish larva to touch stimuli.

Protocol

1. Touch-evoked Response Assay Preparation of 2 dpf Embryos for Touch-evoked Response Assay Ensure that the time of day at which the test is conducted is consistent between experiments because activity can vary dramatically throughout the day. ​NOTE: The experiment should be performed blinded and the order of testing randomized to minimize experimental artifacts. Assign the fish strains a number, which is unknown to the individual carrying out the experime…

Materials

90 mm Petri Dishes  Pacific Laboratory Products PT S90001
Tweezers, style 8  ProSciTech T04-821
Incubator  Thermoline Scientific  TEI-43L
High Speed Camera  Baumer HXC20
StreamPix5  NorPix Step 1.2.3
Temperature Control Unit  Viewpoint
http://www.randomization.com  N/A Steps 1.1.2

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Citar este artigo
Escape Response Assay: A Method to Study the Response of Zebrafish Larva to Touch Stimuli. J. Vis. Exp. (Pending Publication), e20173, doi: (2023).

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