UV Irradiation: A Method to Integrate Extrachromosomal Arrays
Published: April 30, 2023
Abstract
Source: Mariol, M. C et al. A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome. J. Vis. Exp. (2013).
Microinjection of transgenes usually results in C. elegans strains with large extrachromosomal arrays, which result in variable expression and transmission of the transgene. UV Irradiation can induce chromosomal integration of the array and more stable transgene transmission.
Protocol
This protocol is an excerpt from Mariol et al, A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome, J. Vis. Exp. (2013). 1. UV Irradiation and Recovery of Transgenic Worms Worm irradiation: Pick 30-100 fluore…
Representative Results
Table 1. Comparison of the standard and improved protocols for extrachromosomal arrays integration into the C. elegans genome. Two nonintegrated transgenes transmitting at about the same frequency were integrated using the standard or the improved protocol. The main difference lies in the fact that in the standard protocol a screen of the proge…
Materials
| U.V. cross-linker | Fischer Scientific | Wavelength 254 nm | |
| Microscope SteREO Discovery V8 | Carl Zeiss, Inc. | ||
| Petri dishes, 60 mm | CML | BPS55E6 | |
| Platinium wire 0.25 mm | Alfa Aesar | 6/4/7440 | To make a pick for worms |
Citar este artigo
UV Irradiation: A Method to Integrate Extrachromosomal Arrays. J. Vis. Exp. (Pending Publication), e20169, doi: (2023).