C. elegans Muscle Area Measurements: A Standardized Method to Quantify Striated Muscle Morphology
Published: April 30, 2023
Abstract
Source: Teoh, J. S. et al. Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans. J. Vis. Exp. (2019).
C. elegans have two types of muscles, single sarcomere and multiple sarcomere, or striated muscles. This video describes the arrangement of the striated body wall muscles, which are responsible for a worm’s locomotion and includes a sample protocol to measure muscle morphology using image analysis software.
Protocol
This protocol is excerpted from Teoh et al, Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans, J. Vis. Exp. (2019). 1. Quantifying body wall muscle structure Fluorescence imaging of body wall muscle structure</…
Materials
| Confocal microscope | Leica | TCS SP8 | Inverted platform |
| Fluorescence microscope | Carl Zeiss AG | Zeiss Axio Imager M2 | |
| Glass coverslips #1 | Thermo scientifique | MENCS22221GP | |
| Glass coverslips #1.5 | Zeiss | 474030-9000-000 | Made by SCHOTT |
| Glass slides | Thermo scientifique | MENS41104A/40 |
Citar este artigo
C. elegans Muscle Area Measurements: A Standardized Method to Quantify Striated Muscle Morphology. J. Vis. Exp. (Pending Publication), e20165, doi: (2023).