Swimming-Induced Paralysis (SWIP) Assay: A Method to Quantify Dopamine-Mediated Locomotion in C. elegans
Published: April 30, 2023
Abstract
Source: Kudumala, S., et al. Swimming Induced Paralysis to Assess Dopamine Signaling in Caenorhabditis elegans. J. Vis. Exp. (2019).
Here, we introduce an assay to measure SWIP, a behavioral phenotype of C. elegans that occurs during vigorous motion like swimming. The example protocol discusses both manual and automated analysis approaches.
Protocol
The following protocol is an excerpt from Kudumala et al, Swimming Induced Paralysis to Assess Dopamine Signaling in Caenorhabditis elegans, J. Vis. Exp. (2019). SWIP NOTE: We describe the manual method of assessing SWIP in wild-type worms treated with AMPH. We als…
Materials
| Amphetamine | Sigma | 51-63-8 | |
| Depression glass spot plate | Corning | Corning, Inc. 722085 | |
| Eye lash | |||
| Graphing and statistical software | Prism | Graphpad 5 | |
| Osmometer | Advanced Instruments, Inc | Model 3320 | |
| Spreadsheet | MS office | Microsoft Excel | |
| Stereo Microscope | Zeiss | Model tlb3. 1 stemi2000 | |
| Sucrose | Sigma-Aldrich | RB=S5016 | |
| Superglue | Loctite | 1647358 .14 oz. | |
| SwimR sofware | 10.18129/B9.bioc.SwimR | ||
| Tracker 2 | Worm Tracker 2.0 | www.mrc-lmb.cam.ac.uk/wormtracker/ | |
| Video recording software | Virtualdub | http://www.virtualdub.org/ |
Citar este artigo
Swimming-Induced Paralysis (SWIP) Assay: A Method to Quantify Dopamine-Mediated Locomotion in C. elegans. J. Vis. Exp. (Pending Publication), e20137, doi: (2023).