Feeding Test: Assessment of Feeding Preferences in Drosophila melanogaster
Published: April 30, 2023
Abstract
Source: Bovier, T. F., et al. Methods to Test Endocrine Disruption in Drosophila melanogaster. J. Vis. Exp. (2019).
This video describes a feeding assay for fruit flies in which colored food is used to monitor feeding and the effect added compounds may have on feeding activity. The example protocol tests the effects of several endocrine disrupting chemicals (EDC).
Protocol
This protocol is an excerpt from Bovier et al., Methods to Test Endocrine Disruption in Drosophila melanogaster, J. Vis. Exp. (2019). 1. Feeding Assay NOTE: This assay is recommended to test if the presence of the selected EDC in the medium could affect feeding of flies. Put 15 young flies in vials containing Cornmeal medium (CM) supplemented with different concentrations of the selected …
Materials
| 17α-Ethinylestradiol | Sigma | E4876-1G | |
| Agar for Drosophila medium | BIOSIGMA | 789148 | |
| Bisphenol A | Sigma | 239658-50G | |
| Bisphenol AF | Sigma | 90477-100MG | |
| Cornmeal | CA' BIANCA | ||
| Diethyl ether | Sigma | ||
| Drosophila Vials | BIOSIGMA | 789008 | 25 mm x 95 mm |
| Drosophila Vials | BIOSIGMA | 789009 | 29 mm x 95 mm |
| Drosophila Vials | Kaltek | 187 | 22 mm x 63 mm |
| Ethanol | FLUKA | 2860 | |
| Etherizer | Crafts | cylindrical glass container with a cotton plug | |
| Hand blender Pimmy | Ariete | food processor | |
| Instant Success yeast | ESKA | Powdered yeast | |
| Red dye no. 40 | SIGMA | 16035 | |
| Stereomicroscope with LED lights | Leica | S4E |
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Feeding Test: Assessment of Feeding Preferences in Drosophila melanogaster. J. Vis. Exp. (Pending Publication), e20105, doi: (2023).