Drosophila melanogaster Ovary Dissection: A Technique for Ex Vivo Tissue Preparation
Published: April 30, 2023
Abstract
Source: Parker, D. J., et al. Studying Mitochondrial Structure and Function in Drosophila Ovaries. J. Vis. Exp. (2017).
This video describes the general anatomy of the Drosophila ovary and features a method of dissecting ovaries for live or fixed imaging.
Protocol
This protocol is an excerpt from Parker et al., Studying Mitochondrial Structure and Function in Drosophila Ovaries, J. Vis. Exp. (2017). 1. Preparation of Drosophila (the tools required are depicted in Figure 1A) For any of the experiments described, collect Drosophila (maintained at room temperature, or 25 °C) within 5 days of eclosion and place them in a vial filled with 5 …
Representative Results
Figure 1: Experimental Plan and Preparation. (A) Tools used in the methods described: A. Fly vial; B. Insect dissecting medium; C. Paraformaldehyde; D. Fly brush; E. Dissecting dish; F. Cover glass; G. Microfuge tube. H. Glass-bottomed dish; I. Glass slide; J. 1,000-µL micropipette; K. 200-µL micropipette; L. 2.5-µL micropipette; M. Teasing needle with a bent tip (tip …
Materials
| Grace's Media (Insect Dissecting Medium) | Fisher Scientific | 30611031-2 | |
| 41 Paraformaldehyde AQ | Electronic Microscopy Sciences | 50-259-99 | |
| PolyLysine | MP Biomedicals | ICN15017625 | |
| Fly Vials | Fisher Scientific | AS-515 | |
| Fly Vial Plugs | Fisher Scientific | AS273 | |
| Jazzmix Drosophila food (Drosophila food) | Fisher Scientific | AS153 | |
| Azer Scientific EverMark Select Microscope Slides | Fisher Scientific | 22-026-252 | |
| Microscope Cover Glass | Fisher Scientific | 12-542-B | |
| Mat Tek Corp Glass Bottom Mircrowell Dish | Fisher Scientific | P35G-0-14-C | |
| Active Dried Yeast | Fisher Scientific | ICN10140001 | |
| Dumont #5 Forceps | Fine Science Technologies | 11251-20 | |
| Moria Nickel Plated Pin Holder | Fine Science Technologies | 26016-12 | |
| Minutien Pins | Fine Science Technologies | 26002-15 | |
| MYFP ( w[*]; P{w[+mC]=sqh-EYFP-Mito}3 ) | Bloomington Stock Center | 7194 | |
| Fly Pad | Fly stuff | 59-118 | |
| Blowgun | Fly stuff | 54-104 | |
| Blowgun needle | Flystuff | 54-119 | |
| Dissecting Microscope | Carl Zeiss | Stemi 2000 |
Citar este artigo
Drosophila melanogaster Ovary Dissection: A Technique for Ex Vivo Tissue Preparation. J. Vis. Exp. (Pending Publication), e20101, doi: (2023).