Mouse Headpost Implantation: A Method for Head Fixation in Rodents

Published: April 30, 2023

Abstract

Source: França de Barros, F., et al. Long-term Sensory Conflict in Freely Behaving Mice. J. Vis. Exp. (2019).

This video shows a headpost implantation surgery in murine model. The headpost is used not only to head-fix and therefore restrain the mouse during experimentation, but also to securely attach a head device in freely moving animals.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Headpost Implantation Surgery NOTE: Ensure the use of sterile instruments and arrange surgery and recovery in different zones. Once mastered, the implantation procedure lasts about 30 min. For analgesia, 30 min before the beginning of the surgery, subcutaneously inject bupren…

Materials

Blunt scissors FST 14079-10
Catalyst V Sun Medical, Japan LX22 Parkell bio-materials, Kit n°S380
Dentalon Plus Heraeus 37041
Green activator Sun Medical, Japan VE-1 Parkell bio-materials, Kit n°S380
Monomer Sun Medical, Japan MF-1 Parkell bio-materials, Kit n°S380
Ocrygel TvmLab 10779 Ophtalmic vet ointment
Polymer L-type clear (cement) Sun Medical, Japan TT12F Parkell bio-materials, Kit n°S380

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Mouse Headpost Implantation: A Method for Head Fixation in Rodents. J. Vis. Exp. (Pending Publication), e20090, doi: (2023).

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