Maturation Profiling of Human Monocyte-Derived Dendritic Cells (mo-DCs) - JoVE Wordpress Development

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Imunologia e Infecção

人单核细胞来源的树突状细胞（mo-DC）的成熟谱分析

Published: October 20, 2023

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Vanessa C. C. Luz², Zélia Silva², Patrícia Sobral^2,3, Ankit Tanwar⁵, Rachel L. Paterson, Paula A. Videira^2,7

¹Associate Laboratory i4HB – Institute for Health and Bioeconomy, NOVA School of Science and Technology,Universidade NOVA de Lisboa, ²UCIBIO – Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa, ³LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia,Universidade NOVA de Lisboa, ⁴Department of Microbiology and Immunology,Albert Einstein College of Medicine, ⁵Department of Oncology,Albert Einstein College of Medicine, ⁶Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark,Zona Industrial da Gandra, ⁷CDG & Allies – Professionals and Patient Associations International Network (CDG & Allies – PPAIN), Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa

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Keywords: Maturation Profiling Monocyte-derived Dendritic Cells Mo-DCs Antibody Staining Fluorescence Conjugated Antibodies PBS Paraformaldehyde Flow Cytometry MHC-I MHC-II CD80 CD86 Sialidase Treatment

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Maturation Profiling of Human Monocyte-Derived Dendritic Cells (mo-DCs). J. Vis. Exp. (Pending Publication), e200352, doi:10.3791/200352 (2023).

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