Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues

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Biologia

Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE

Published: July 28, 2023

doi:

10.3791/200322

Lucas Stalder, Fabian Koechl, Kerstin Hahn, Marc Sultan, Megana K. Prasad

¹Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

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Keywords: Single Nuclei Sucrose Gradient Cleanup Nuclei Suspension SCS Centrifugation Propidium Iodide Staining Cell Counter Single Nuclei RNA Sequencing Gene Counts UMI Counts Mitochondrial Content Cellular Proportions Technical Variability

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Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues. J. Vis. Exp. (Pending Publication), e200322, doi:10.3791/200322 (2023).

Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE

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