JOVE JOURNAL

Biologia

Showing 1,561 - 1,572 in 3,287 videos.

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Journal (Biology)

A Cell-Free Assay Using Xenopus laevis Embryo Extracts to Study Mechanisms of Nuclear Size Regulation

Lisa J. Edens¹, Daniel L. Levy¹

¹Department of Molecular Biology,University of Wyoming

Journal (Biology)

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Haseong Kim¹, Kil Koang Kwon¹, Wonjae Seong¹, Seung-Goo Lee^1,2

¹Synthetic Biology & Bioengineering Research Center,Korea Research Institute of Bioscience and Biotechnology, ²Biosystems and Bioengineering Program,University of Science and Technology, Daejeon, South Korea

Journal (Biology)

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Sally M. McFall¹, Mário F. Neto¹, Jennifer L. Reed¹, Robin L. Wagner²

¹Center for Innovation in Global Health Technologies (CIGHT), Department of Biomedical Engineering,Northwestern University, ²Pritzker School of Medicine,The University of Chicago

Journal (Biology)

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

Huazheng Liang*^1,2, Bassem Akladios*¹, Cesar P. Canales*¹, Richard Francis³, Edna H. Hardeman¹, Annemiek Beverdam¹

¹The School of Medical Sciences,University of New South Wales Australia, ²Neuroscience Research Australia, ³Biomedical Imaging Facility,University of New South Wales Australia

Journal (Biology)

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans

Beomseok Seo¹, Junho Lee^1,2,3

¹Institute of Molecular Biology and Genetics (IMBG),Seoul National University, ²Department of Biological Sciences,Seoul National University, ³Department of Biophysics and Chemical Biology,Seoul National University

Journal (Biology)

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Dorrelyn P. Martin¹, Jharna Miya¹, Julie W. Reeser¹, Sameek Roychowdhury^1,2

¹Department of Internal Medicine, Division of Medical Oncology, Comprehensive Cancer Center,The Ohio State University, ²Department of Pharmacology,The Ohio State University

Journal (Biology)

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Emanuel G. Worst¹, Matthias P. Exner², Alessandro De Simone², Marc Schenkelberger¹, Vincent Noireaux³, Nediljko Budisa², Albrecht Ott¹

¹Department of Experimental Physics,Saarland University, ²Institute of Chemistry,Technische Universität Berlin, ³School of Physics and Astronomy,University of Minnesota

Journal (Biology)

FRET Imaging in Three-dimensional Hydrogels

Amalie E. Donius*¹, Sylvain V. Bougoin*^1,3, Juan M. Taboas^1,2

¹Department of Oral Biology, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine,University of Pittsburgh, ²Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine,University of Pittsburgh, ³Laerdal AS

Journal (Biology)

Assessment of Myofilament Ca²⁺ Sensitivity Underlying Cardiac Excitation-contraction Coupling

Zai Hao Zhao¹, Chun Li Jin¹, Ji Hyun Jang¹, Yu Na Wu¹, Sung Joon Kim¹, Hong Hua Jin², Lan Cui², Yin Hua Zhang^1,2,3

¹Department of Physiology & Biomedical Sciences, Ischemic/hypoxic Disease Institute,Seoul National University College of Medicine, ²Yan Bian University Hospital, ³Institute of Cardiovascular Sciences,University of Manchester

Journal (Biology)

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Clarisse van der Feltz¹, Daniel Pomeranz Krummel^1,2

¹Department of Biochemistry,Brandeis University, ²Winship Cancer Institute,Emory University School of Medicine

Journal (Biology)

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Paulina J. Stanczyk¹, F. Anthony Lai¹, Spyros Zissimopoulos¹

¹Wales Heart Research Institute,Cardiff University School of Medicine, College of Biomedical and Life Sciences

Journal (Biology)

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Mulugeta Seneshaw¹, Faridoddin Mirshahi¹, Hae-Ki Min¹, Amon Asgharpour¹, Shervin Mirshahi¹, Kalyani Daita¹, Sherry Boyett¹, Prasanna K. Santhekadur², Michael Fuchs¹, Arun J. Sanyal¹

¹Division of Gastroenterology, Hepatology and Nutrition,Virginia Commonwealth University, ²McGuire Veterans Affairs Medical Center,Research BC-101

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