JOVE JOURNAL

Bioquímica

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Journal (Biochemistry)

Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry

Myndert Claasen, Zornitsa Kofinova, Matteo Contino, Weston B. Struwe⁴

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Analysis of the Microfluidics Coupled Mass Photometry Data to Investigate Low-Affinity Protein-Protein Interactions

Myndert Claasen, Zornitsa Kofinova, Matteo Contino, Weston B. Struwe⁴

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery,University of Oxford

Journal (Biochemistry)

Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET

Constanza Enriquez-Toledo*¹, Cesar A. Ponce-Diego*¹, Cesar L. Cuevas-Velazquez

¹Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

Journal (Biochemistry)

Yeast Cell Preparation for FRET Assay for Hyperosmotic Stress Analysis

Constanza Enriquez-Toledo*¹, Cesar A. Ponce-Diego*¹, Cesar L. Cuevas-Velazquez

¹Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

Journal (Biochemistry)

Isothermal Titration Calorimetry for Evaluating PROTACS-Induced Ternary Complex Formation

Wei Jiang, Holly Soutter

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

Wei Jiang, Holly Soutter

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Biolayer Interferometry (BLI) for Evaluating PROTACS-Induced Ternary Complex Formation

Wei Jiang, Holly Soutter

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Surface Plasmon Resonance (SPR) for Evaluating PROTACS-Induced Ternary Complex Formation

Wei Jiang, Holly Soutter

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation

Trinity Cookis, Paul Sauer³, Simon Poepsel⁵, Bong-Gyoon Han, Dominik A. Herbst^2,6, Robert Glaeser, Eva Nogales^2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

Journal (Biochemistry)

Designing Streptavidin Affinity Grids for Cryo-Electron Microscopy

Trinity Cookis, Paul Sauer³, Simon Poepsel⁵, Bong-Gyoon Han, Dominik A. Herbst^2,6, Robert Glaeser, Eva Nogales^2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

Journal (Biochemistry)

Sample Preparation for In Situ Cryotomography of Mammalian Cells

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen, Thomas Lidahl³, Luiza Mendonça

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Preparing Electron Microscopy (EM) Grids for Cellular Cryotomography

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen, Thomas Lidahl³, Luiza Mendonça

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

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