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Journal (Cancer Research)

In Vitro Drug Validation to Assay Ion Channel Function in Cancer Cells

Laura Kallay¹, Vaibhavkumar S. Gawali¹, Donatien Kamdem Toukam¹, Debanjan Bhattacharya¹, Andrew Jenkins², Soma Sengupta³, Daniel A. Pomeranz Krummel³

¹Department of Neurology and Rehabilitation Medicine, Division of Neuro-Oncology,University of Cincinnati College of Medicine, ²Department of Pharmaceutical Sciences, School of Pharmacy,University of Saint Joseph, ³The Vontz Center for Molecular Studies,University of Cincinnati College of Medicine

Journal (Cancer Research)

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Gisele O. L. Rodrigues¹, WenQing Li¹, Sarah D. Cramer^1,2,3, Hila Y. Winer¹, Tu Chun Hsu^1,2,3, Timothy Gower⁴, Julie A. Hixon¹, Scott K. Durum¹

¹Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute,National Institutes of Health, ²Comparative Biomedical Scientist Training Program,NIH, ³Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory,Michigan State University, ⁴NCI-Frederick Laboratory Animal Sciences Program

Journal (Cancer Research)

Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma

Maodi Wu^1,3, Yang Liu², Yi Zhao³, Yuyao Zhang⁴, Liansi Huang³, Qijun Du³, Ting Zhang^1,3, Zhihui Zhong^1,3, Han Luo², Kai Xiao^1,3

¹Precision Medicine Research Center, Sichuan Provincial Key Laboratory of Precision Medicine, National Clinical Research Center for Geriatrics, West China Hospital,Sichuan University, ²Department of Thyroid Surgery, West China Hospital,Sichuan University, ³Sichuan Kangcheng Biotech Co., ⁴Sichuan Cancer Hospital

Journal (Cancer Research)

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Nicolas Huyghe¹, Elena Benidovskaya¹, Simon Beyaert¹, Aurélie Daumerie⁴, Finoula Maestre Osorio¹, Frank Aboubakar Nana^2,3, Caroline Bouzin⁴, Marc Van den Eynde^1,5

¹Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO,Université Catholique de Louvain (UCLouvain), ²Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU),Université Catholique de Louvain (UCLouvain), ³Division of Pneumology, Cliniques Universitaires St-Luc,Université Catholique de Louvain (UCLouvain), ⁴IREC Imaging Platform,Université Catholique de Louvain (UCLouvain), ⁵Institut Roi Albert II, Department of Medical Oncology and Gastroenterology,Cliniques Universitaires St-Luc

Journal (Cancer Research)

Multiplex Immunofluorescence Staining and Image Analysis

Nicolas Huyghe¹, Elena Benidovskaya¹, Simon Beyaert¹, Aurélie Daumerie⁴, Finoula Maestre Osorio¹, Frank Aboubakar Nana^2,3, Caroline Bouzin⁴, Marc Van den Eynde^1,5

¹Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO,Université Catholique de Louvain (UCLouvain), ²Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU),Université Catholique de Louvain (UCLouvain), ³Division of Pneumology, Cliniques Universitaires St-Luc,Université Catholique de Louvain (UCLouvain), ⁴IREC Imaging Platform,Université Catholique de Louvain (UCLouvain), ⁵Institut Roi Albert II, Department of Medical Oncology and Gastroenterology,Cliniques Universitaires St-Luc

Journal (Cancer Research)

Pancreatic Tissue Dissection to Isolate Viable Single Cells

Oshri Yosefov-Levi¹, Sharona Tornovsky¹, Oren Parnas¹

¹The Lautenberg Center for Immunology and Cancer Research, The Institute for Medical Research Israel-Canada, Faculty of Medicine,The Hebrew University of Jerusalem

Journal (Cancer Research)

Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts

¹Molecular Epidemiology and Predictive Tumor Markers Group, Area 3,Ramón y Cajal Health Research Institute (IRYCIS), ²The Biomedical Research Network in Cancer (CIBERONC), ³Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678),Ramón y Cajal Health Research Institute (IRYCIS), ⁴Faculty of Medicine,University of Alcalá de Henares, ⁵Institute of Tissue Medicine and Pathology,University of Bern, ⁶Department of Molecular Oncology, Cancer Research Institute,Biomedical Research Center of the Slovak Academy of Sciences, ⁷Experimental Oncology, Molecular Oncology Program,Centro Nacional de Investigaciones Oncológicas (CNIO), ⁸Department of Surgical Oncology, National Cancer Institute,Slovak Medical University, ⁹Pancreatic and Biliopancreatic Surgery Unit,Hospital Universitario Ramón y Cajal, ¹⁰Department of Pathology,Hospital Universitario Ramón y Cajal, ¹¹Department of Radiation Oncology,Hospital Universitario Ramón y Cajal, ¹²Department of Cancer,Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), ¹³Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3,IRYCIS

Journal (Cancer Research)

Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue

¹Molecular Epidemiology and Predictive Tumor Markers Group, Area 3,Ramón y Cajal Health Research Institute (IRYCIS), ²The Biomedical Research Network in Cancer (CIBERONC), ³Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678),Ramón y Cajal Health Research Institute (IRYCIS), ⁴Faculty of Medicine,University of Alcalá de Henares, ⁵Institute of Tissue Medicine and Pathology,University of Bern, ⁶Department of Molecular Oncology, Cancer Research Institute,Biomedical Research Center of the Slovak Academy of Sciences, ⁷Experimental Oncology, Molecular Oncology Program,Centro Nacional de Investigaciones Oncológicas (CNIO), ⁸Department of Surgical Oncology, National Cancer Institute,Slovak Medical University, ⁹Pancreatic and Biliopancreatic Surgery Unit,Hospital Universitario Ramón y Cajal, ¹⁰Department of Pathology,Hospital Universitario Ramón y Cajal, ¹¹Department of Radiation Oncology,Hospital Universitario Ramón y Cajal, ¹²Department of Cancer,Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), ¹³Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3,IRYCIS

Journal (Cancer Research)

Processing the Single-Cell Shear Assay Imaging Data

Luke J. Holen*¹, Killian Onwudiwe*¹, Julian Najera¹, Maksym Zarodniuk¹, John D. Obayemi², Winston O. Soboyejo², Meenal Datta¹

¹Department of Aerospace and Mechanical Engineering,University of Notre Dame, ²Departments of Mechanical and Biomedical Engineering,Worcester Polytechnic Institute

Journal (Cancer Research)

Shear Assay Protocol for the Determination of Single-Cell Material Properties

Luke J. Holen*¹, Killian Onwudiwe*¹, Julian Najera¹, Maksym Zarodniuk¹, John D. Obayemi², Winston O. Soboyejo², Meenal Datta¹

¹Department of Aerospace and Mechanical Engineering,University of Notre Dame, ²Departments of Mechanical and Biomedical Engineering,Worcester Polytechnic Institute

Journal (Cancer Research)

Microfluidic Shear Apparatus Setup for Single-Cell Fluid Shear Assay

Luke J. Holen*¹, Killian Onwudiwe*¹, Julian Najera¹, Maksym Zarodniuk¹, John D. Obayemi², Winston O. Soboyejo², Meenal Datta¹

¹Department of Aerospace and Mechanical Engineering,University of Notre Dame, ²Departments of Mechanical and Biomedical Engineering,Worcester Polytechnic Institute

Journal (Cancer Research)

Performing Single-Cell Shear Assay and Imaging

Luke J. Holen*¹, Killian Onwudiwe*¹, Julian Najera¹, Maksym Zarodniuk¹, John D. Obayemi², Winston O. Soboyejo², Meenal Datta¹

¹Department of Aerospace and Mechanical Engineering,University of Notre Dame, ²Departments of Mechanical and Biomedical Engineering,Worcester Polytechnic Institute

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