JOVE JOURNAL

Bioquímica

Showing 385 - 396 in 759 videos.

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Ritika Tewari*¹, Savannah J. West*^1,2, Bieerkehazi Shayahati, Askar M. Akimzhanov

¹Department of Biochemistry and Molecular Biology,McGovern Medical School at UT Health, ²MD Anderson UT Health Graduate School

Journal (Biochemistry)

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

Jessica A. Espino, Lisa M. Jones

¹Department of Pharmaceutical Sciences,University of Maryland

Journal (Biochemistry)

Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins

David J. Speca, Elva Diaz

¹Department of Pharmacology,UC Davis School of Medicine

Journal (Biochemistry)

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Maria Lucia Pigazzini*^1,2, Christian Gallrein*¹, Manuel Iburg*¹, Gabriele Kaminski Schierle, Janine Kirstein⁴

¹Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin, ²NeuroCure Cluster of Excellence,Charité - Universitätsmedizin Berlin, ³Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology,University of Cambridge, ⁴Cell Biology,University of Bremen

Journal (Biochemistry)

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Alexandria Slayden, Kelsey North, Shivantika Bisen, Alex M. Dopico, Anna N. Bukiya, Avia Rosenhouse-Dantsker

¹Department of Pharmacology, Addiction Science and Toxicology,The University of Tennessee HSC, ²Department of Chemistry,University of Illinois at Chicago

Journal (Biochemistry)

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Karel Bezstarosti, Lennart van der Wal, Jeroen A. A. Demmers

¹Proteomics Center,Erasmus University Medical Center

Journal (Biochemistry)

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson’s Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Ying Fan*¹, Francesca Tonelli*¹, Shalini Padmanabhan, Marco A.S. Baptista, Lindsey Riley, Danielle Smith, Connie Marras, Andrew Howden, Dario R. Alessi, Esther Sammler⁶

¹MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, ²The Michael J. Fox Foundation for Parkinson's Research, ³Department of Medical and Molecular Genetics,Indiana University School of Medicine, ⁴Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital,University of Toronto, ⁵Division of Cell Signalling and Immunology, School of Life Sciences,University of Dundee, ⁶Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School,University of Dundee

Journal (Biochemistry)

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

Filip Pamula², Jonas Mühle, Alain Blanc, Rony Nehmé, Patricia C. Edwards, Christopher G. Tate, Ching-Ju Tsai

¹Laboratory of Biomolecular Research,Paul Scherrer Institute, ²Department of Biology,ETH Zürich, ³Center for Radiopharmaceutical Sciences,Paul Scherrer Institute, ⁴Laboratory of Molecular Biology,Medical Research Council

Journal (Biochemistry)

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Hayley Abbiss^2,3, Joel P. A. Gummer⁴, Michael Francki⁶, Robert D. Trengove

¹Research and Innovation,Murdoch University, ²Centre for Digital Agriculture,Curtin University, ³Centre for Integrative Metabolomics and Computational Biology, School of Science,Edith Cowan University, ⁴Centre for Computational and System Medicine, Health Futures Institute,Murdoch University, ⁵Department of Primary Industries and Regional Development, ⁶State Agricultural Biotechnology Centre,Murdoch University

Journal (Biochemistry)

Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence

Damian Jacenik, Marta Zielińska, Sylwia Michlewska, Jakub Fichna, Wanda M. Krajewska

¹Department of Cytobiochemistry, Faculty of Biology and Environmental Protection,University of Lodz, ²Department of Biochemistry, Faculty of Medicine,Medical University of Lodz, ³Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection,University of Lodz

Journal (Biochemistry)

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

Emily E. Chea, Aimee Rinas, Jessica A. Espino, Lisa M. Jones

¹Department of Pharmaceutical Sciences,University of Maryland Baltimore, ²AIT Bioscience

Journal (Biochemistry)

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

Manel Zeghal, Geneviève Laroche, Patrick M. Giguère²

¹Department of Biochemistry, Microbiology and Immunology,University of Ottawa, ²Brain and Mind Research Institute,University of Ottawa

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