University of São Paulo View Institution's Website 4 articles published in JoVE Engineering Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators Olivier Morin1, Jianli Liu1, Kun Huang1,2, Felippe Barbosa3, Claude Fabre1, Julien Laurat1 1Laboratoire Kastler Brossel, Université Pierre et Marie Curie, Ecole Normale Supérieure, CNRS, 2State Key Laboratory of Precision Spectroscopy, East China Normal University, 3Instituto de Física, Universidade de São Paulo We describe the reliable generation of non-Gaussian states of traveling optical fields, including single-photon states and coherent state superpositions, using a conditional preparation method operated on the non-classical light emitted by optical parametric oscillators. Type-I and type-II phase-matched oscillators are considered and common procedures, such as the required frequency filtering or the high-efficiency quantum state characterization by homodyning, are detailed. Biology An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans Bruno B. Queliconi1, Alicia J. Kowaltowski1, Keith Nehrke2 1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, 2Department of Medicine, Nephrology Division, University of Rochester Medical Center, School of Medicine and Dentistry A protocol is described that uses anoxia/starvation in C. elegans to model ischemia/reperfusion. Functional outcomes include increased mortality, visible abnormalities in GFP-labeled neuronal processes, and impaired behavioral responses that require neuronal function. Biology Determining the Contribution of the Energy Systems During Exercise Guilherme G. Artioli1, Rômulo C. Bertuzzi2, Hamilton Roschel1,3, Sandro H. Mendes1, Antonio H. Lancha Jr.1, Emerson Franchini4 1Laboratory of Applied Nutrition, School of Physical Education and Sport, University of Sao Paulo, 2Aerobic Performance Research Group, School of Physical Education and Sport, University of Sao Paulo, 3Laboratory of Neuromuscular Adaptations to Strength Training, School of Physical Education and Sport, University of Sao Paulo, 4Martial Arts and Combat Sports Research Group, School of Physical Education and Sport, University of Sao Paulo This protocol allows researchers focused on exercise and sports sciences to determine the relative contribution of three different energy systems to the total energy expenditure during a large variety of exercises. Medicine Vascular Occlusion Training for Inclusion Body Myositis: A Novel Therapeutic Approach Bruno Gualano1,2, Carlos Ugrinowitsch1, Manoel Neves Jr.2, Fernanda R. Lima2, Ana Lúcia S. Pinto2, Gilberto Laurentino1, Valmor A.A. Tricoli1, Antonio H. Lancha Jr.1, Hamilton Roschel1,2 1School of Physical Education and Sport, University of São Paulo, 2Division of Rheumatology, School of Medicine, University of São Paulo The present article presents the details pertaining to the application of resistance training associated to vascular occlusion in IBM patients.